![]() But for what it's worth, the dilution factor for 1 mL stock + 99 mL water is:Īmount transferred / (amount transferred + amount water added) =īelow is an applet to practice finding dilution factors, and also to determine how much water to add to achieve a given dilution. It's possible to write an algebraic expression for the dilution factor, but it's almost more trouble than it's worth, because it sounds so complex. What matters is how much you transfer and how much water you add. The easiest method is to make a series of 1 in 10 dilutions. To produce 10-fold serial dilutions, pipette 1 ml from the milk and buffer mixture into the first 9 ml dilution blank. A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration. Notice that it really doesn't matter how much of the original stock you started with, as long as you had enough to put 1 mL into the new container. Label dilution blanks with the dilution value, remember the mixed liquid sample is already at a (10-1) dilution, so your next dilutions will be (10-2, 10-3), if transferring 1 ml into 9 ml dilution blanks. A number of spread plates is needed because the exact number of live bacteria in the sample is usually unknown. 10-2 to 10-8 ) is normally performed on the sample culture and spread plates created from the dilutions. Typically, a serial 1:1 dilution (dilution factor 2 in DI. Here's what a dilution factor of 0.01 looks like on the lab bench: Figure 1: Serial dilution series and plating. We could also have saidĪs you've probably guessed, this works exactly the same whether you're talking about caffeine or meningicocci. ![]() The 1 represents the amount of sample added. To complete a tenfold dilution, the ratio must be 1:10. ![]() Log Dilutions A log dilution is a tenfold dilution, meaning the concentration is decreased by a multiple of ten. When you're thinking about dilution, it helps to simplify your actions into dilution factors. understanding of dilutions and should be used as a guideline, not a replacement for laboratory procedures. ![]()
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